The 450 mile tour across Scotland was set up in memory of Alyson Porter, a fellow biker who died from breast cancer last year.
The charity run departs from Kilmarmock and will stop in Oban, Inverness and Fort William before finishing in George Square, Glasgow on Monday.
Last year the ride raised 26,500 and this year they are aiming to surpass that amount.
Event organiser Shirley Ann Barnes, who started organised the ride after her friend died last July, said: We are all really looking forward to the bike ride. It will be hard going but I know we will all have a really great time.”
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